Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
The use of CRISPRa in bacteria lacks predictive rules for identifying effective gRNA target sites. Here the authors define features of bacterial promoters that impose stringent requirements on effective sites.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2020-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-15454-y |