New Approach for Detection of Normal Alternative Splicing Events and Aberrant Spliceogenic Transcripts with Long-Range PCR and Deep RNA Sequencing

New Approach for Detection of Normal Alternative Splicing Events and Aberrant Spliceogenic Transcripts with Long-Range PCR and Deep RNA Sequencing

RNA sequencing is a promising technique for detecting normal and aberrant RNA isoforms. Here, we present a new single-gene, straightforward 1-day hands-on protocol for detection of splicing alterations with deep RNA sequencing from blood. We have validated our method’s accuracy by detecting previous...

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Bibliographic Details
Main Authors: Vita Šetrajčič Dragoš, Vida Stegel, Ana Blatnik, Gašper Klančar, Mateja Krajc, Srdjan Novaković
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Biology
Subjects:
splicing
RNA sequencing
alternative splicing
germline variant
spliceogenic variant
alternative transcript
Online Access:https://www.mdpi.com/2079-7737/10/8/706

Internet

https://www.mdpi.com/2079-7737/10/8/706

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