Using RNA-Seq to assemble a rose transcriptome with more than 13,000 full-length expressed genes and to develop the WagRhSNP 68k Axiom SNP array for rose (Rosa L.)

Using RNA-Seq to assemble a rose transcriptome with more than 13,000 full-length expressed genes and to develop the WagRhSNP 68k Axiom SNP array for rose (Rosa L.)

In order to develop a versatile and large SNP array for rose, we set out to mine ESTs from diverse sets of rose germplasm. For this RNA-Seq libraries containing about 700 million reads were generated from tetraploid cut and garden roses using Illumina paired-end sequencing, and from diploid Rosa mul...

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Bibliographic Details
Main Authors: Carole F S Koning-Boucoiran, G Danny Esselink, Mirjana eVukosavljev, Wendy P C Van 't Westende, Virginia W Gitonga, Frans Andries Krens, Roeland E Voorrips, W Eric Van de Weg, Dietmar eSchulz, Thomas eDebener, Paul eArens, Marinus J M eSmulders
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-04-01
Series:Frontiers in Plant Science
Subjects:
Rosa
Transcriptome
genotyping array
Assembly
SNP array
tetraploid
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00249/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00249/full

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