Development and application of a Bacillus anthracis protective antigen domain-1 in-house ELISA for the detection of anti-protective antigen antibodies in cattle in Zambia.

In Zambia, anthrax outbreaks among cattle are reported on nearly an annual basis. Presently, there is a lack of serological assays and information to develop an anthrax management and control strategy. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant protecti...

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Bibliographic Details
Main Authors: Manyando Simbotwe, Daisuke Fujikura, Miyuki Ohnuma, Ryosuke Omori, Yoshikazu Furuta, Geoffrey Munkombwe Muuka, Bernard Mudenda Hang'ombe, Hideaki Higashi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6193699?pdf=render