Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM + FLIM)

Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM + FLIM)

We present a new microscope integrating super-resolved imaging using structured illumination microscopy (SIM) with wide-field optically sectioned fluorescence lifetime imaging (FLIM) to provide optical mapping of molecular function and its correlation with biological nanostructure below the conventi...

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Bibliographic Details
Main Authors: Frederik Görlitz, David S. Corcoran, Edwin A. Garcia Castano, Birgit Leitinger, Mark A. A. Neil, Christopher Dunsby, Paul M. W. French
Format: Article
Language:English
Published: MDPI AG 2017-07-01
Series:Photonics
Subjects:
fluorescence lifetime microscopy
structured illumination microscopy
Förster energy transfer
collagen receptor
DNA
Online Access:https://www.mdpi.com/2304-6732/4/3/40

Internet

https://www.mdpi.com/2304-6732/4/3/40

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