Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions.

Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions.

Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved. In this study, we have developed a ribosomal binding site (RBS) switching strategy for direct cloning...

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Bibliographic Details
Main Authors: Yangbo Hu, Lipeng Feng, Yunlong Li, Yong Zhang, Pei Lu, Simon Rayner, Shiyun Chen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3503710?pdf=render

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http://europepmc.org/articles/PMC3503710?pdf=render

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