Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.

Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.

The ability to obtain accurate and reproducible data using quantitative real-time Polymerase Chain Reaction (RT-qPCR) is limited by the process of data normalization. The use of 'housekeeping' or 'reference' genes is the most common technique used to normalize RT-qPCR data. Howev...

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Bibliographic Details
Main Authors: Kristen C Thomas, Xi Fiona Zheng, Francia Garces Suarez, Joanna M Raftery, Kate G R Quinlan, Nan Yang, Kathryn N North, Peter J Houweling
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3921188?pdf=render

Internet

http://europepmc.org/articles/PMC3921188?pdf=render

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