Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges

Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges

<p>Abstract</p> <p>Background</p> <p>The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications. Recent experiments indica...

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Bibliographic Details
Main Authors: Mai Timo, Kayser Jona, Ehler Oliver, Naiser Thomas, Michel Wolfgang, Ott Albrecht
Format: Article
Language:English
Published: BMC 2008-05-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/8/48

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http://www.biomedcentral.com/1472-6750/8/48

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