Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike protein

Summary: This protocol describes an integrated approach for analyzing site-specific N- and O-linked glycosylation of SARS-CoV-2 spike protein by mass spectrometry. Glycoproteomics analyzes intact glycopeptides to examine site-specific microheterogeneity of glycoproteins. Glycomics provides structura...

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Bibliographic Details
Main Authors: Katelyn E. Rosenbalm, Michael Tiemeyer, Lance Wells, Kazuhiro Aoki, Peng Zhao
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672030201X