Expression, Immobilization and Enzymatic Properties of Glutamate Decarboxylase Fused to a Cellulose-Binding Domain

Expression, Immobilization and Enzymatic Properties of Glutamate Decarboxylase Fused to a Cellulose-Binding Domain

Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamic acid to gamma-aminobutyric acid (GABA), was fused to the cellulose-binding domain (CBD) and a linker of Trichoderma harzianum endoglucanase II. To prevent proteolysis of the fusion protein, th...

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Bibliographic Details
Main Authors: Eun Gyo Lee, Hongweon Lee, Chunsuk Kim, Juwhan Lee, Hyeokwon Lee, Hyemin Park, Jungoh Ahn, Joon-Ki Jung
Format: Article
Language:English
Published: MDPI AG 2011-12-01
Series:International Journal of Molecular Sciences
Subjects:
GAD
cellulose-binding domain
fusion protein
immobilization
Online Access:http://www.mdpi.com/1422-0067/13/1/358/

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http://www.mdpi.com/1422-0067/13/1/358/

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