A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis
<p>Abstract</p> <p>Background</p> <p>The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production. This limits the use of long oligonucleotides in assays w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-08-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/7/49 |