Protoplast fusion between Pleurotus ostreatus and P. djamor
Protoplast fusion between Pleurotus ostreatus and P. djamor was carried out by isolating protoplasts from 4-day-old monokaryotic mycelia cultured on malt extract broth. The mycelia were then agitated at 100 rpm for 2 h with 9 mg Lysing Enzyme (Sigma L-1412) in 1 ml osmotic stabilizer (0.6 M MgSO4·7H...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Prince of Songkla University
2005-09-01
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Series: | Songklanakarin Journal of Science and Technology (SJST) |
Subjects: | |
Online Access: | http://www.sjst.psu.ac.th/journal/27-5-pdf/05-protoplast-fusion.pdf |