Protoplast fusion between Pleurotus ostreatus and P. djamor

Protoplast fusion between Pleurotus ostreatus and P. djamor was carried out by isolating protoplasts from 4-day-old monokaryotic mycelia cultured on malt extract broth. The mycelia were then agitated at 100 rpm for 2 h with 9 mg Lysing Enzyme (Sigma L-1412) in 1 ml osmotic stabilizer (0.6 M MgSO4·7H...

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Bibliographic Details
Main Authors: Chaninan Pornsuriya, Pannee Dhitaphichit
Format: Article
Language:English
Published: Prince of Songkla University 2005-09-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://www.sjst.psu.ac.th/journal/27-5-pdf/05-protoplast-fusion.pdf