Long DCL4-substrate dsRNAs efficiently induce RNA interference in plant cells

Abstract RNA interference (RNAi) is induced by the direct transfer of double-stranded RNAs (dsRNAs) into protoplasts prepared from Arabidopsis thaliana seedlings. In this protoplast RNAi system, we compared the efficacies of various-sized dsRNAs (between 21 and 139 nucleotides [nt]) for inducing RNA...

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Bibliographic Details
Main Authors: Sayaka Kakiyama, Midori Tabara, Yuki Nishibori, Hiromitsu Moriyama, Toshiyuki Fukuhara
Format: Article
Language:English
Published: Nature Publishing Group 2019-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-019-43443-9