The Scopoletin-HRP Fluorimetric Determination of H2O2 in Seawaters—A Plea for the Two-Stage Protocol

A single solution protocol has been widely used for the fluorimetric determination of H2O2 in natural waters by its bleaching of the fluorescing scopoletin in the presence of the enzyme horseradish peroxidase (HRP). In this protocol, the reaction between scopoletin and H2O2 in the sample and the sub...

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Bibliographic Details
Main Authors: Man Wu, George T. F. Wong, Yao-Chu Wu
Format: Article
Language:English
Published: MDPI AG 2017-12-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/1/1/4