CAR T Cell Generation by piggyBac Transposition from Linear Doggybone DNA Vectors Requires Transposon DNA-Flanking Regions

CAR T Cell Generation by piggyBac Transposition from Linear Doggybone DNA Vectors Requires Transposon DNA-Flanking Regions

CD19-specific chimeric antigen receptor (CAR19) T cells, generated using viral vectors, are an efficacious but costly treatment for B cell malignancies. The nonviral piggyBac transposon system provides a simple and inexpensive alternative for CAR19 T cell production. Until now, piggyBac has been pla...

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Bibliographic Details
Main Authors: David C. Bishop, Lisa Caproni, Kavitha Gowrishankar, Michal Legiewicz, Kinga Karbowniczek, John Tite, David J. Gottlieb, Kenneth P. Micklethwaite
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
Chimeric antigen receptor
CAR
piggyBac transposon
doggybone
non-viral vector
minimal DNA vector
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050120300127

Internet

http://www.sciencedirect.com/science/article/pii/S2329050120300127

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