Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates

• Main Authors: Takeshi Imanishi, Satoshi Obika, Futaba Makimura, Ayako Orita, Tomohisa Osaki, Noritsugu Hosoki, Tetsuya Kodama, Kosuke Ramon Ito
• Format: Article
• Language: English
• Published: MDPI AG 2011-12-01
• Series: Molecules
• Subjects: bridged nucleic acids; DNA sensing; P3’→N5’ phosphoramidate
• Online Access: http://www.mdpi.com/1420-3049/16/12/10695/
• ISSN: 1420-3049

Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3’→N5’ phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whether duplex formation also induced conformational strain, we examined the reactivity of the oligonucleotides with a P-N linkage in the presence of single-stranded templates, and compared these reactions to those with dsDNA templates. P-N oligonucleotides that are cleaved upon duplex formation could be used as probes to detect single-stranded nucleic acids.