Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change

Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change

Summary: Protein-peptide interactions are involved in many fundamental cellular functions and constitute promising drug targets. Here, we provide a detailed protocol for the cost-effective preparation of a cellulose-based solid support for synthesis of nanoscale to micromolar-scale peptide libraries...

Full description

Bibliographic Details
Main Authors: Clemens Schulte, Vladimir Khayenko, Amit Jean Gupta, Hans Michael Maric
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Biophysics
Biotechnology and bioengineering
High Throughput Screening
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003129
id doaj-83421419d4a440e29a3ce482a6ee0562
record_format Article
spelling doaj-83421419d4a440e29a3ce482a6ee05622021-09-19T04:59:51ZengElsevierSTAR Protocols2666-16672021-09-0123100605Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity changeClemens Schulte0Vladimir Khayenko1Amit Jean Gupta2Hans Michael Maric3Rudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Wuerzburg, Josef-Schneider-Str. 2, 97080 Wuerzburg, Germany; Biocenter, Department of Biotechnology and Biophysics, University of Wuerzburg, 97074 Wuerzburg, Germany; Corresponding authorRudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Wuerzburg, Josef-Schneider-Str. 2, 97080 Wuerzburg, Germany; Biocenter, Department of Biotechnology and Biophysics, University of Wuerzburg, 97074 Wuerzburg, GermanyNanotemper Technologies GmbH, Flößergasse 4, 81369 Munich, GermanyRudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Wuerzburg, Josef-Schneider-Str. 2, 97080 Wuerzburg, Germany; Biocenter, Department of Biotechnology and Biophysics, University of Wuerzburg, 97074 Wuerzburg, Germany; Corresponding authorSummary: Protein-peptide interactions are involved in many fundamental cellular functions and constitute promising drug targets. Here, we provide a detailed protocol for the cost-effective preparation of a cellulose-based solid support for synthesis of nanoscale to micromolar-scale peptide libraries. Their subsequent use for high-throughput protein interaction screening as well as affinity determination in solution provides binding data for thousands of unique peptides with a turnover of 1 to 2 weeks, thereby facilitating in vitro assessment and development of high-affinity binders.For complete details on the use and execution of this protocol, please refer to Schulte et al., (2020)http://www.sciencedirect.com/science/article/pii/S2666166721003129BiophysicsBiotechnology and bioengineeringHigh Throughput ScreeningMolecular/Chemical ProbesProtein Biochemistry
collection DOAJ
language English
format Article
sources DOAJ
author Clemens Schulte
Vladimir Khayenko
Amit Jean Gupta
Hans Michael Maric
spellingShingle Clemens Schulte
Vladimir Khayenko
Amit Jean Gupta
Hans Michael Maric
Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
STAR Protocols
Biophysics
Biotechnology and bioengineering
High Throughput Screening
Molecular/Chemical Probes
Protein Biochemistry
author_facet Clemens Schulte
Vladimir Khayenko
Amit Jean Gupta
Hans Michael Maric
author_sort Clemens Schulte
title Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
title_short Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
title_full Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
title_fullStr Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
title_full_unstemmed Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
title_sort low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-09-01
description Summary: Protein-peptide interactions are involved in many fundamental cellular functions and constitute promising drug targets. Here, we provide a detailed protocol for the cost-effective preparation of a cellulose-based solid support for synthesis of nanoscale to micromolar-scale peptide libraries. Their subsequent use for high-throughput protein interaction screening as well as affinity determination in solution provides binding data for thousands of unique peptides with a turnover of 1 to 2 weeks, thereby facilitating in vitro assessment and development of high-affinity binders.For complete details on the use and execution of this protocol, please refer to Schulte et al., (2020)
topic Biophysics
Biotechnology and bioengineering
High Throughput Screening
Molecular/Chemical Probes
Protein Biochemistry
url http://www.sciencedirect.com/science/article/pii/S2666166721003129
work_keys_str_mv AT clemensschulte lowcostsynthesisofpeptidelibrariesandtheiruseforbindingstudiesviatemperaturerelatedintensitychange
AT vladimirkhayenko lowcostsynthesisofpeptidelibrariesandtheiruseforbindingstudiesviatemperaturerelatedintensitychange
AT amitjeangupta lowcostsynthesisofpeptidelibrariesandtheiruseforbindingstudiesviatemperaturerelatedintensitychange
AT hansmichaelmaric lowcostsynthesisofpeptidelibrariesandtheiruseforbindingstudiesviatemperaturerelatedintensitychange
_version_ 1717376222512545792

Similar Items