Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen.

BACKGROUND: Group II introns are RNA enzymes that splice themselves from pre-mRNA transcripts. Most bacterial group II introns harbour an open reading frame (ORF), coding for a protein with reverse transcriptase, maturase and occasionally DNA binding and endonuclease activities. Some ORF-containing...

Full description

Bibliographic Details
Main Authors: Christine Ritlop, Caroline Monat, Benoit Cousineau
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3410883?pdf=render