Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from <i>Phanerochaete</i> <i>chrysosporium</i> Using the <i>E. coli</i>-Expression System

Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from <i>Phanerochaete</i> <i>chrysosporium</i> Using the <i>E. coli</i>-Expression System

Manganese peroxidases (MnP) from the white-rot fungi <i>Phanerochaete chrysosporium</i> catalyse the oxidation of Mn<sup>2+</sup> to Mn<sup>3+</sup>, a strong oxidizer able to oxidize a wide variety of organic compounds. Different approaches have been used to unra...

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Bibliographic Details
Main Authors: Angel De La Cruz Pech-Canul, Javier Carrillo-Campos, María de Lourdes Ballinas-Casarrubias, Rosa Lidia Solis-Oviedo, Selena Karina Hernández-Rascón, León Raúl Hernández-Ochoa, Néstor Gutiérrez-Méndez, Antonio García-Triana
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
manganese peroxidase
mnp1
recombinant
codon-optimization
<i>phanerochaete</i>
<i>escherichia coli</i>
inteins
chitin binding domain (cbd)
protein purification
affinity chromatography
Online Access:https://www.mdpi.com/1422-0067/21/2/416

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