Islet Purification Method Using Large Bottles Effectively Achieves High Islet Yield from Pig Pancreas

Porcine islets are a promising resource for xenotransplantation. However, low efficacy of islet isolation because of their marked fragility remains a problem. Recently we found that the standard purification method using COBE 2991 cell processor (COBE) with Ficoll density gradient solution damaged i...

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Bibliographic Details
Main Authors: Masayuki Shimoda, Hirofumi Noguchi, Yasutaka Fujita, Morihito Takita, Tetsuya Ikemoto, Daisuke Chujo, Bashoo Naziruddin Ph.D., Marlon F. Levy, Naoya Kobayashi, Paul A. Grayburn, Shinichi Matsumoto M.D., Ph.D.
Format: Article
Language:English
Published: SAGE Publishing 2012-03-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/096368911X605411