Multiple imputation and direct estimation for qPCR data with non-detects

Multiple imputation and direct estimation for qPCR data with non-detects

Abstract Background Quantitative real-time PCR (qPCR) is one of the most widely used methods to measure gene expression. An important aspect of qPCR data that has been largely ignored is the presence of non-detects: reactions failing to exceed the quantification threshold and therefore lacking a mea...

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Bibliographic Details
Main Authors: Valeriia Sherina, Helene R. McMurray, Winslow Powers, Harmut Land, Tanzy M. T. Love, Matthew N. McCall
Format: Article
Language:English
Published: BMC 2020-11-01
Series:BMC Bioinformatics
Subjects:
Gene expression
Quantitative real-time PCR (qPCR)
Missing not at random (MNAR)
Non-detects
Direct estimation
Multiple imputation
Online Access:http://link.springer.com/article/10.1186/s12859-020-03807-9

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http://link.springer.com/article/10.1186/s12859-020-03807-9

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