An improved reverse transcription-polymerase chain reaction method to study apolipoprotein gene expression in Caco-2 cells.
We report a method for the detection and analysis of apolipoprotein B mRNA using the thermostable enzyme rTth to perform coupled reverse transcription-polymerase chain reaction (RT-PCR) amplification. This method, which is at least a 100-fold more sensitive than traditional RT-PCR, was used to exami...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1994-02-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520412222 |