Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath.

A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results i...

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Bibliographic Details
Main Authors: Sandeep K Kasoji, Samantha G Pattenden, Ewa P Malc, Chatura N Jayakody, James K Tsuruta, Piotr A Mieczkowski, William P Janzen, Paul A Dayton
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4505845?pdf=render