Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites

Heterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their...

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Main Authors:	Antonella Virgilio, Luciano Mayol, Michela Varra, Aldo Galeone, Veronica Esposito
Format:	Article
Language:	English
Published:	Hindawi Limited 2015-01-01
Series:	Journal of Chemistry
Online Access:	http://dx.doi.org/10.1155/2015/473051

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spelling	doaj-7ede7e25476f4101bac84499484bc5b92020-11-25T00:29:09ZengHindawi LimitedJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/473051473051Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity SitesAntonella Virgilio0Luciano Mayol1Michela Varra2Aldo Galeone3Veronica Esposito4Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyHeterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their ability to fold in G-quadruplex structures through a combination of gel electrophoresis, circular dichroism, and UV spectroscopy techniques. In H1 and H2 inversions of polarity sites have been introduced to control the strand direction in the loop regions. Collected data suggest that all modified sequences are able to fold in chair-like G-quadruplexes mimicking the original TBA structure.http://dx.doi.org/10.1155/2015/473051
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Antonella Virgilio Luciano Mayol Michela Varra Aldo Galeone Veronica Esposito
spellingShingle	Antonella Virgilio Luciano Mayol Michela Varra Aldo Galeone Veronica Esposito Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites Journal of Chemistry
author_facet	Antonella Virgilio Luciano Mayol Michela Varra Aldo Galeone Veronica Esposito
author_sort	Antonella Virgilio
title	Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites
title_short	Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites
title_full	Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites
title_fullStr	Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites
title_full_unstemmed	Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites
title_sort	unusual chair-like g-quadruplex structures: heterochiral tba analogues containing inversion of polarity sites
publisher	Hindawi Limited
series	Journal of Chemistry
issn	2090-9063 2090-9071
publishDate	2015-01-01
description	Heterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their ability to fold in G-quadruplex structures through a combination of gel electrophoresis, circular dichroism, and UV spectroscopy techniques. In H1 and H2 inversions of polarity sites have been introduced to control the strand direction in the loop regions. Collected data suggest that all modified sequences are able to fold in chair-like G-quadruplexes mimicking the original TBA structure.
url	http://dx.doi.org/10.1155/2015/473051
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Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites

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