Pooling skin swabs does not inhibit qPCR detection of amphibian chytrid infection.

Immediate and reliable pathogen detection in large numbers of samples is essential in wildlife disease monitoring and is often realized by DNA-based techniques. Pooling samples increases processing efficiency and reduces processing costs, and has been suggested as a viable technique for quantitative...

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Bibliographic Details
Main Authors: Joana Sabino-Pinto, An Martel, Frank Pasmans, Sebastian Steinfartz, Miguel Vences
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0214405