Detection of an amplification bias associated to Leuconostocaceae family with a universal primer routinely used for monitoring microbial community structures within food products

Detection of an amplification bias associated to Leuconostocaceae family with a universal primer routinely used for monitoring microbial community structures within food products

Abstract Objectives Sequencing of 16S rDNA V3–V4 region is widely applied for food community profiling. However, two different universal forward primers (named here MUYZER-primer1 and KLINDWORTH-primer2) targeting an identical conservative sequence upstream of the V3 region of 16S rRNA gene, and onl...

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Bibliographic Details
Main Authors: Simon Poirier, Olivier Rué, Gwendoline Coeuret, Marie-Christine Champomier-Vergès, Valentin Loux, Stéphane Chaillou
Format: Article
Language:English
Published: BMC 2018-11-01
Series:BMC Research Notes
Subjects:
V3 universal primer
16S rDNA amplification bias
MiSeq pair-end sequencing
Food microbiota
Leuconostoc genus
Online Access:http://link.springer.com/article/10.1186/s13104-018-3908-2

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http://link.springer.com/article/10.1186/s13104-018-3908-2

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