Identification of Endogenous Control miRNAs for RT-qPCR in T-Cell Acute Lymphoblastic Leukemia

Identification of Endogenous Control miRNAs for RT-qPCR in T-Cell Acute Lymphoblastic Leukemia

Optimal endogenous controls enable reliable normalization of microRNA (miRNA) expression in reverse-transcription quantitative PCR (RT-qPCR). This is particularly important when miRNAs are considered as candidate diagnostic or prognostic biomarkers. Universal endogenous controls are lacking, thus ca...

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Bibliographic Details
Main Authors: Monika Drobna, Bronisława Szarzyńska-Zawadzka, Patrycja Daca-Roszak, Maria Kosmalska, Roman Jaksik, Michał Witt, Małgorzata Dawidowska
Format: Article
Language:English
Published: MDPI AG 2018-09-01
Series:International Journal of Molecular Sciences
Subjects:
miRNA (microRNA)
T-cell acute lymphoblastic leukemia (T-ALL)
normalization of miRNA expression in RT-qPCR
endogenous controls
reference genes
tissue analysis
cell lines
Online Access:http://www.mdpi.com/1422-0067/19/10/2858

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http://www.mdpi.com/1422-0067/19/10/2858

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