Studies to Prevent Degradation of Recombinant Fc-Fusion Protein Expressed in Mammalian Cell Line and Protein Characterization

Studies to Prevent Degradation of Recombinant Fc-Fusion Protein Expressed in Mammalian Cell Line and Protein Characterization

Clipping of recombinant proteins is a major issue in animal cell cultures. A recombinant Fc-fusion protein, VEGFR1(D1–D3)-Fc expressed in CHOK1SV GS-KO cells was observed to be undergoing clippings in lab scale cultures. Partial cleaving of expressed protein initiated early on in cell culture and wa...

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Bibliographic Details
Main Authors: Sanjukta Chakrabarti, Colin J. Barrow, Rupinder K. Kanwar, Venkata Ramana, Jagat R. Kanwar
Format: Article
Language:English
Published: MDPI AG 2016-06-01
Series:International Journal of Molecular Sciences
Subjects:
Fc fusion protein
VEGFR1(D1–D3)-Fc
CHOK1SV GS-KO
clipping
proteolysis
inhibition
stable
folded
biological activity
Online Access:http://www.mdpi.com/1422-0067/17/6/913

Internet

http://www.mdpi.com/1422-0067/17/6/913

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