SpCas9- and LbCas12a-Mediated DNA Editing Produce Different Gene Knockout Outcomes in Zebrafish Embryos

SpCas9- and LbCas12a-Mediated DNA Editing Produce Different Gene Knockout Outcomes in Zebrafish Embryos

CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein) genome editing is a powerful technology widely used in current genetic research. In the most simple and straightforward way it can be applied for a gene knockout resulting from repair errors, induced by...

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Bibliographic Details
Main Authors: Darya A. Meshalkina, Aleksei S. Glushchenko, Elana V. Kysil, Igor V. Mizgirev, Andrej Frolov
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Genes
Subjects:
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), Cas9 (CRISPR associated protein 9)
Cas12a (CRISPR associated protein 12a)
Cpf1 (CRISPR-associated endonuclease in Prevotella and Francisella 1)
zebrafish
gene knockout
repair outcome
Online Access:https://www.mdpi.com/2073-4425/11/7/740

Internet

https://www.mdpi.com/2073-4425/11/7/740

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