Generation and validation of PAX7 reporter lines from human iPS cells using CRISPR/Cas9 technology

Generation and validation of PAX7 reporter lines from human iPS cells using CRISPR/Cas9 technology

Directed differentiation of iPS cells toward various tissue progenitors has been the focus of recent research. Therefore, generation of tissue-specific reporter iPS cell lines provides better understanding of developmental stages in iPS cells. This technical report describes an efficient strategy fo...

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Bibliographic Details
Main Authors: Jianbo Wu, Samuel D. Hunt, Haipeng Xue, Ying Liu, Radbod Darabi
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Subjects:
PAX7
Human iPS cells
GFP reporter
Cas9
dCas9
CRISPR
Cas9 nickase
Knock-in
Homologous recombination
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000131

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http://www.sciencedirect.com/science/article/pii/S1873506116000131

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