Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells

Summary: This protocol is intended as a guide for implementing or refining the usage of the air-liquid interface (ALI) model system to generate airway mucociliary tissue in vitro. We present a streamlined protocol for isolating the stem cells from inferior nasal turbinates of donors, allowing for a...

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Bibliographic Details
Main Authors: Vincent Manna, Salvatore Caradonna
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004883