On measuring miRNAs after transient transfection of mimics or antisense inhibitors.

The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To achieve this there is widespread use of both exogenous double-stranded miRNA mimics for transient over-expression, and single stranded antisense RNAs (antimiRs) for miRNA inhibition. The success of these manip...

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Bibliographic Details
Main Authors: Daniel W Thomson, Cameron P Bracken, Jan M Szubert, Gregory J Goodall
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3554668?pdf=render