Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system

Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system

Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human...

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Main Authors: Yongli Shan, Lishi Ma, Cong Zhang, Yanqi Zhang, Jingyuan Zhang, Lu Xiao, Yanxing Wei, Yanhong Yu
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120301860
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spelling doaj-7a7303beeaf94c4bad664602cfaafdff2020-11-25T03:57:33ZengElsevierStem Cell Research1873-50612020-08-0147101885Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 systemYongli Shan0Lishi Ma1Cong Zhang2Yanqi Zhang3Jingyuan Zhang4Lu Xiao5Yanxing Wei6Yanhong Yu7Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Corresponding authors at: Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China (Y. Shan and Y. Yu).Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, ChinaCAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaCAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaCAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaPlacenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, ChinaPlacenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, ChinaPlacenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Corresponding authors at: Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China (Y. Shan and Y. Yu).Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.http://www.sciencedirect.com/science/article/pii/S1873506120301860
collection DOAJ
language English
format Article
sources DOAJ
author Yongli Shan
Lishi Ma
Cong Zhang
Yanqi Zhang
Jingyuan Zhang
Lu Xiao
Yanxing Wei
Yanhong Yu
spellingShingle Yongli Shan
Lishi Ma
Cong Zhang
Yanqi Zhang
Jingyuan Zhang
Lu Xiao
Yanxing Wei
Yanhong Yu
Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
Stem Cell Research
author_facet Yongli Shan
Lishi Ma
Cong Zhang
Yanqi Zhang
Jingyuan Zhang
Lu Xiao
Yanxing Wei
Yanhong Yu
author_sort Yongli Shan
title Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
title_short Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
title_full Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
title_fullStr Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
title_full_unstemmed Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
title_sort generation of two rnf2 homozygous knockout human embryonic stem cell lines by crispr/cas9 system
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-08-01
description Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.
url http://www.sciencedirect.com/science/article/pii/S1873506120301860
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AT lishima generationoftwornf2homozygousknockouthumanembryonicstemcelllinesbycrisprcas9system
AT congzhang generationoftwornf2homozygousknockouthumanembryonicstemcelllinesbycrisprcas9system
AT yanqizhang generationoftwornf2homozygousknockouthumanembryonicstemcelllinesbycrisprcas9system
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