Direct long-read RNA sequencing identifies a subset of questionable exitrons likely arising from reverse transcription artifacts

Direct long-read RNA sequencing identifies a subset of questionable exitrons likely arising from reverse transcription artifacts

Abstract Resistance to CD19-directed immunotherapies in lymphoblastic leukemia has been attributed, among other factors, to several aberrant CD19 pre-mRNA splicing events, including recently reported excision of a cryptic intron embedded within CD19 exon 2. While “exitrons” are known to exist in hun...

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Bibliographic Details
Main Authors: Laura Schulz, Manuel Torres-Diz, Mariela Cortés-López, Katharina E. Hayer, Mukta Asnani, Sarah K. Tasian, Yoseph Barash, Elena Sotillo, Kathi Zarnack, Julian König, Andrei Thomas-Tikhonenko
Format: Article
Language:English
Published: BMC 2021-06-01
Series:Genome Biology
Subjects:
Long-read sequencing
Oxford Nanopore Technologies
Alternative splicing
mRNA isoforms
Exitrons
Reverse transcription
Online Access:https://doi.org/10.1186/s13059-021-02411-1

Internet

https://doi.org/10.1186/s13059-021-02411-1

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