Single enzyme molecule assay with time resolution using capillary electrophoresis
The continuous flow assay is a capillary electrophoresis-based methodology for the simultaneous measurement of the catalytic rate and electrophoretic mobility of individual molecules of the enzyme β-galactosidase. The method also provides time resolution. This method was used to measure the distribu...
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Format: | Article |
Language: | English |
Published: |
De Gruyter
2013-05-01
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Series: | Reviews in Analytical Chemistry |
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Online Access: | https://doi.org/10.1515/revac-2012-0040 |