Single enzyme molecule assay with time resolution using capillary electrophoresis

The continuous flow assay is a capillary electrophoresis-based methodology for the simultaneous measurement of the catalytic rate and electrophoretic mobility of individual molecules of the enzyme β-galactosidase. The method also provides time resolution. This method was used to measure the distribu...

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Bibliographic Details
Main Author: Craig Douglas B.
Format: Article
Language:English
Published: De Gruyter 2013-05-01
Series:Reviews in Analytical Chemistry
Subjects:
Online Access:https://doi.org/10.1515/revac-2012-0040