Spatial multi-omics sequencing for fixed tissue via DBiT-seq

Summary: This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells...

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Bibliographic Details
Main Authors: Graham Su, Xiaoyu Qin, Archibald Enninful, Zhiliang Bai, Yanxiang Deng, Yang Liu, Rong Fan
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002392