An asymmetric protoplast fusion and screening method for generating celeriac cybrids

Abstract Celeriac F1 hybrid seed production is currently complicated due to the instability of cytoplasmic male sterile lines. To develop alternative alloplasmic CMS lines, an asymmetric protoplast fusion and hybrid screening methodology was established. Celeriac suspension cells protoplasts were us...

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Bibliographic Details
Main Authors: Silvia Bruznican, Tom Eeckhaut, Johan Van Huylenbroeck, Ellen De Keyser, Hervé De Clercq, Danny Geelen
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-83970-y