Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme

Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme

Abstract Background Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these enzymes need a native conformation stabilized by disulfide bonds, a...

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Bibliographic Details
Main Authors: Ekaterina Jalomo-Khayrova, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero, Marco A. Ramos
Format: Article
Language:English
Published: BMC 2018-04-01
Series:BMC Biotechnology
Subjects:
Recombinant protein
Amebic cysteine protease
Expression and purification
Cytosolic oxidative folding
Proteolytic activity
Enzyme inhibition
Online Access:http://link.springer.com/article/10.1186/s12896-018-0429-y

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http://link.springer.com/article/10.1186/s12896-018-0429-y

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