Analysis of the Localization of Fluorescent PpROP1 and PpROP-GEF4 Fusion Proteins in Moss Protonemata Based on Genomic “Knock-In” and Estradiol-Titratable Expression

Analysis of the Localization of Fluorescent PpROP1 and PpROP-GEF4 Fusion Proteins in Moss Protonemata Based on Genomic “Knock-In” and Estradiol-Titratable Expression

Tip growth of pollen tubes, root hairs, and apical cells of moss protonemata is controlled by ROP (Rho of plants) GTPases, which were shown to accumulate at the apical plasma membrane of these cells. However, most ROP localization patterns reported in the literature are based on fluorescent protein...

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Bibliographic Details
Main Authors: Aude Le Bail, Sylwia Schulmeister, Pierre-François Perroud, Maria Ntefidou, Stefan A. Rensing, Benedikt Kost
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Plant Science
Subjects:
XFP tagging
genomic knock-in
estradiol-inducible expression
Physcomitrella patens
ROP signaling
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2019.00456/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2019.00456/full

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