An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films

Abstract Increased interest in poly(ethylene terephthalate) (PET)-degrading enzymes (PETases) have generated efforts to find mutants with improved catalytic activity and thermostability. Here, we present a simple and fast method to determine relative enzyme kinetics through bulk absorbance measureme...

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Bibliographic Details
Main Authors: En Ze Linda Zhong-Johnson, Christopher A. Voigt, Anthony J. Sinskey
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-79031-5