Quantitative Flow Cytometry to Understand Population Heterogeneity in Response to Changes in Substrate Availability in Escherichia coli and Saccharomyces cerevisiae Chemostats

Microbial cells in bioprocesses are usually described with averaged parameters. But in fact, single cells within populations vary greatly in characteristics such as stress resistance, especially in response to carbon source gradients. Our aim was to introduce tools to quantify population heterogenei...

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Bibliographic Details
Main Authors: Anna-Lena Heins, Ted Johanson, Shanshan Han, Luisa Lundin, Magnus Carlquist, Krist V. Gernaey, Søren J. Sørensen, Anna Eliasson Lantz
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-08-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00187/full