Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules

Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules

SUMMARY: WNT signaling is crucial for embryonic development, adult tissue homeostasis, and injury repair. The poor biophysical characteristics of WNTs and their lack of receptor selectivity have hindered their use as research tools or potential therapeutics. We have developed a platform to generate...

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Main Authors: Hui Chen, Chenggang Lu, Sung-Jin Lee, Yang Li
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300307
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spelling doaj-76f479f8aff04133927ea2ff8110ab1c2020-11-25T04:07:38ZengElsevierSTAR Protocols2666-16672020-06-0111100043Protocol to Generate and Characterize Potent and Selective WNT Mimetic MoleculesHui Chen0Chenggang Lu1Sung-Jin Lee2Yang Li3Surrozen Inc., 171 Oyster Point Blvd, Suite 400, South San Francisco, CA 94080, USASurrozen Inc., 171 Oyster Point Blvd, Suite 400, South San Francisco, CA 94080, USASurrozen Inc., 171 Oyster Point Blvd, Suite 400, South San Francisco, CA 94080, USASurrozen Inc., 171 Oyster Point Blvd, Suite 400, South San Francisco, CA 94080, USA; Corresponding authorSUMMARY: WNT signaling is crucial for embryonic development, adult tissue homeostasis, and injury repair. The poor biophysical characteristics of WNTs and their lack of receptor selectivity have hindered their use as research tools or potential therapeutics. We have developed a platform to generate potent, soluble, selective WNT mimetics with drug-like properties for both research and therapeutic applications. To help researchers adapt and expand on this platform, we describe these protocols and key considerations in generating and studying WNT mimetics.For complete details on the use and execution of this protocol, please refer to Chen et al., 2020.http://www.sciencedirect.com/science/article/pii/S2666166720300307
collection DOAJ
language English
format Article
sources DOAJ
author Hui Chen
Chenggang Lu
Sung-Jin Lee
Yang Li
spellingShingle Hui Chen
Chenggang Lu
Sung-Jin Lee
Yang Li
Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
STAR Protocols
author_facet Hui Chen
Chenggang Lu
Sung-Jin Lee
Yang Li
author_sort Hui Chen
title Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_short Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_full Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_fullStr Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_full_unstemmed Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_sort protocol to generate and characterize potent and selective wnt mimetic molecules
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-06-01
description SUMMARY: WNT signaling is crucial for embryonic development, adult tissue homeostasis, and injury repair. The poor biophysical characteristics of WNTs and their lack of receptor selectivity have hindered their use as research tools or potential therapeutics. We have developed a platform to generate potent, soluble, selective WNT mimetics with drug-like properties for both research and therapeutic applications. To help researchers adapt and expand on this platform, we describe these protocols and key considerations in generating and studying WNT mimetics.For complete details on the use and execution of this protocol, please refer to Chen et al., 2020.
url http://www.sciencedirect.com/science/article/pii/S2666166720300307
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AT chengganglu protocoltogenerateandcharacterizepotentandselectivewntmimeticmolecules
AT sungjinlee protocoltogenerateandcharacterizepotentandselectivewntmimeticmolecules
AT yangli protocoltogenerateandcharacterizepotentandselectivewntmimeticmolecules
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