ConservedPrimers 2.0: A high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery
<p>Abstract</p> <p>Background</p> <p>In some genomic applications it is necessary to design large numbers of PCR primers in exons flanking one or several introns on the basis of orthologous gene sequences in related species. The primer pairs designed by this target gene...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2009-10-01
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Series: | BMC Bioinformatics |
Online Access: | http://www.biomedcentral.com/1471-2105/10/331 |