Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms

The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submit...

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Main Authors: Alessandra De Cesare, Antonio Parisi, Alex Lucchi, Loredana Capozzi, Angela Bianco, Frederique Pasquali, Gerardo Manfreda
Format: Article
Language:English
Published: PAGEPress Publications 2019-02-01
Series:Italian Journal of Food Safety
Subjects:
Online Access:https://pagepressjournals.org/index.php/ijfs/article/view/7706
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spelling doaj-7660cea23777472185e160cde2653c042020-11-25T01:50:38ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322019-02-017410.4081/ijfs.2018.7706Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farmsAlessandra De Cesare0Antonio Parisi1Alex Lucchi2Loredana Capozzi3Angela Bianco4Frederique Pasquali5Gerardo Manfreda6Department of Agricultural and Food Sciences, Alma Mater Studiorum – University of BolognaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Sezione di Putignano, BariDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of BolognaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Sezione di Putignano, BariIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Sezione di Putignano, BariDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of BolognaDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of Bologna The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitted to DNA extraction. Total DNA was sequenced in the 16S rRNA and reads analysed with MG-RAST to classify the colonising bacteria up to the genus level and compare each taxonomic group in terms of mean relative frequency of abundance in conventional and antibiotic free carcasses. Firmicutes displayed abundances always higher than 38% but did not show statistically significative differences between conventional and antibiotic free carcasses. On the contrary, Bacteroidetes and Actinobacteria were significantly higher in antibiotic free then conventional carcasses (21.57 vs 10.95%; 19.29 vs 12.05%), whereas Proteobacteria were higher in the latter (33.19 vs 19.52%). The genera significantly higher in antibiotic free than conventional carcasses were Chryseobacterium (10.07 vs 1.94%), Rothia (3.08 vs 0.77%) and Micrococcus (1.12 vs 0.16%), while Shewanella was significantly higher in conventional carcasses (1.38 vs 0.26%). Among Firmicutes, the genera significantly higher in conventional carcasses were Ureibacillus (1.45 vs 0.11%) and Bacillus (3.28 vs 0.56%). The higher abundance of Proteobacteria in conventional carcasses might suggest that hygienic conditions in conventional farms are worse than antibiotic free farms. However, from a food safety point of view, Salmonella was not detected in both kinds of carcasses and the Campylobacter mean relative frequency of abundance was always lower than 0.4%. https://pagepressjournals.org/index.php/ijfs/article/view/7706Microbiota, Poultry Carcasses, Microbiological Hazards, Conventional Farms, Antibiotic Free Farms.
collection DOAJ
language English
format Article
sources DOAJ
author Alessandra De Cesare
Antonio Parisi
Alex Lucchi
Loredana Capozzi
Angela Bianco
Frederique Pasquali
Gerardo Manfreda
spellingShingle Alessandra De Cesare
Antonio Parisi
Alex Lucchi
Loredana Capozzi
Angela Bianco
Frederique Pasquali
Gerardo Manfreda
Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
Italian Journal of Food Safety
Microbiota, Poultry Carcasses, Microbiological Hazards, Conventional Farms, Antibiotic Free Farms.
author_facet Alessandra De Cesare
Antonio Parisi
Alex Lucchi
Loredana Capozzi
Angela Bianco
Frederique Pasquali
Gerardo Manfreda
author_sort Alessandra De Cesare
title Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_short Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_full Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_fullStr Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_full_unstemmed Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_sort microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
publisher PAGEPress Publications
series Italian Journal of Food Safety
issn 2239-7132
publishDate 2019-02-01
description The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitted to DNA extraction. Total DNA was sequenced in the 16S rRNA and reads analysed with MG-RAST to classify the colonising bacteria up to the genus level and compare each taxonomic group in terms of mean relative frequency of abundance in conventional and antibiotic free carcasses. Firmicutes displayed abundances always higher than 38% but did not show statistically significative differences between conventional and antibiotic free carcasses. On the contrary, Bacteroidetes and Actinobacteria were significantly higher in antibiotic free then conventional carcasses (21.57 vs 10.95%; 19.29 vs 12.05%), whereas Proteobacteria were higher in the latter (33.19 vs 19.52%). The genera significantly higher in antibiotic free than conventional carcasses were Chryseobacterium (10.07 vs 1.94%), Rothia (3.08 vs 0.77%) and Micrococcus (1.12 vs 0.16%), while Shewanella was significantly higher in conventional carcasses (1.38 vs 0.26%). Among Firmicutes, the genera significantly higher in conventional carcasses were Ureibacillus (1.45 vs 0.11%) and Bacillus (3.28 vs 0.56%). The higher abundance of Proteobacteria in conventional carcasses might suggest that hygienic conditions in conventional farms are worse than antibiotic free farms. However, from a food safety point of view, Salmonella was not detected in both kinds of carcasses and the Campylobacter mean relative frequency of abundance was always lower than 0.4%.
topic Microbiota, Poultry Carcasses, Microbiological Hazards, Conventional Farms, Antibiotic Free Farms.
url https://pagepressjournals.org/index.php/ijfs/article/view/7706
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