Co-fractionation/mass spectrometry to identify protein complexes

Summary: Co-fractionation/mass spectrometry (CF/MS) is a flexible and powerful method to detect physical associations of proteins. CF/MS can be applied to any tissue or organism without the need for protein-specific antibodies or epitope tags. Here, we outline two alternate protocols for MS preparat...

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Bibliographic Details
Main Authors: Claire D. McWhite, Ophelia Papoulas, Kevin Drew, Vy Dang, Janelle C. Leggere, Wisath Sae-Lee, Edward M. Marcotte
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000770