Preparation of MS2 phage-like particles and their use as potential process control viruses for detection and quantification of enteric RNA viruses in different matrices

Preparation of MS2 phage-like particles and their use as potential process control viruses for detection and quantification of enteric RNA viruses in different matrices

The detection and quantification of enteric RNA viruses is based on isolation of viral RNA from the sample followed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). To control the whole process of analysis and in order to guarantee the validity and reliability of results, p...

Full description

Bibliographic Details
Main Authors: Pavel Mikel, Petra Vasickova, Radek Tesarik, Hana Malenovska, Pavel Kulich, Tomas Vesely, Petr Kralik
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-12-01
Series:Frontiers in Microbiology
Subjects:
detection
quantification
RT-qPCR
Isolation
RNA virus
MS2 phage-like particle
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01911/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01911/full

Similar Items