Routine single particle CryoEM sample and grid characterization by tomography

Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Bibliographic Details
Main Authors: Alex J Noble, Venkata P Dandey, Hui Wei, Julia Brasch, Jillian Chase, Priyamvada Acharya, Yong Zi Tan, Zhening Zhang, Laura Y Kim, Giovanna Scapin, Micah Rapp, Edward T Eng, William J Rice, Anchi Cheng, Carl J Negro, Lawrence Shapiro, Peter D Kwong, David Jeruzalmi, Amedee des Georges, Clinton S Potter, Bridget Carragher
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-05-01
Series:eLife
Subjects:
cryoET
single particle
protomo
fiducial-less
air-water
tomography
Online Access:https://elifesciences.org/articles/34257

Internet

https://elifesciences.org/articles/34257

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