Engineering subtilisin proteases that specifically degrade active RAS

Engineering subtilisin proteases that specifically degrade active RAS

Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro...

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Bibliographic Details
Main Authors: Yingwei Chen, Eric A. Toth, Biao Ruan, Eun Jung Choi, Richard Simmerman, Yihong Chen, Yanan He, Ruixue Wang, Raquel Godoy-Ruiz, Harlan King, Gregory Custer, D. Travis Gallagher, David A. Rozak, Melani Solomon, Silvia Muro, David J. Weber, John Orban, Thomas R. Fuerst, Philip N. Bryan
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-01818-7

Internet

https://doi.org/10.1038/s42003-021-01818-7

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