Fixation-induced cell blebbing on spread cells inversely correlates with phosphatidylinositol 4,5-bisphosphate level in the plasma membrane

Fixation-induced cell blebbing on spread cells inversely correlates with phosphatidylinositol 4,5-bisphosphate level in the plasma membrane

While most attention has been focused on physiologically generated blebs, the molecular mechanisms for fixation-induced cell blebbing are less investigated. We show that protein-fixing (e.g. aldehydes and picric acid) but not lipid-stabilizing (e.g. OsO4 and KMnO4) fixatives induce blebbing on sprea...

Full description

Bibliographic Details
Main Authors: Siyuan Zhao, Huanhuan Liao, Meiying Ao, Li Wu, Xiaojun Zhang, Yong Chen
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:FEBS Open Bio
Subjects:
Cell fixation
Cell blebbing
Phosphatidylinositol 4,5-bisphosphate (PIP2)
Human umbilical vein endothelial cells (HUVECs)
Lipid rafts
THP-1-derived macrophages
Online Access:http://www.sciencedirect.com/science/article/pii/S221154631400014X

Internet

http://www.sciencedirect.com/science/article/pii/S221154631400014X

Similar Items