Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research

Abstract Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with d...

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Bibliographic Details
Main Authors: Glenn Vergauwen, Bert Dhondt, Jan Van Deun, Eva De Smedt, Geert Berx, Evy Timmerman, Kris Gevaert, Ilkka Miinalainen, Véronique Cocquyt, Geert Braems, Rudy Van den Broecke, Hannelore Denys, Olivier De Wever, An Hendrix
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-02599-y